LOCUS       sequence-118772-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attaches mCerulean(1-158) to the C-terminus of a 
            protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  TITLE     Unpublished BiFC Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Cerulean(1-158)Stop in pcDNA1/amp
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(141..162)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(402..421)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(622..640)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(623..640)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          724..789
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accgagctcg gatccatggt gagcaagggc gaggagctgt
      121 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      181 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      241 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg
      301 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      361 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      421 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      481 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac gccatcagcg
      541 acaacgtcta tatcaccgcc gacaagcagt aaagatctat cacactggcg gccgctcgag
      601 catgcatcta gagggcccta ttctatagtg tcacctaaat gctagaggat ctttgtgaag
      661 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
      721 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
      781 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgccttt
//
