LOCUS       sequence-118773-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attaches mCerulean(1-158) to the C-terminus of a 
            protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  TITLE     Unpublished BiFC Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Cerulean(1-158)Stop in pcDNA1/amp
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     173..192
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     432..453
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(529..548)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(530..548)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(593..692)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(622..642)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggccgccagt gtgatagatc tttactgctt gtcggcggtg atatagacgt tgtcgctgat
       61 ggcgttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
      121 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
      181 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
      241 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact gcacgcccca
      301 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
      361 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
      421 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
      481 ctcgcccttg ctcaccatgg atccgagctc ggtaccaagc ttgggtctcc ctatagtgag
      541 tcgtattaat ttcgataagc cagtaagcag tgggttctct agttagccag agagctctgc
      601 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
      661 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
      721 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
      781 gccaaaa
//
