LOCUS       sequence-118775-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attaches CFP(1-158) to the N-terminus of a protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: CFP(1-158) in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(83..85,86..88,89..547)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITA"
     primer_bind     complement(128..149)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(389..408)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(606..624)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(607..624)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          708..773
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttgg
       61 taccgagctc ggatccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
      121 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      181 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      241 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag
      301 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      361 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      421 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      481 ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat
      541 caccgccgac aagcagagat ctatcacact ggcggccgct cgagcatgca tctagagggc
      601 cctattctat agtgtcacct aaatgctaga ggatctttgt gaaggaacct tacttctgtg
      661 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
      721 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
      781 cctatggaac tgatgaatgg gagcagtggt ggaatg
//
