LOCUS       sequence-118776-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attaches CFP(1-158) to the N-terminus of a protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: CFP(1-158) in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(14..472,473..475,476..478))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITA"
     primer_bind     153..172
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     412..433
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      475..484
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(515..534)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(516..534)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(579..678)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(608..628)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atctctgctt gtcggcggtg atatagacgt tgtggctgat gtagttgtac tccagcttgt
       61 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
      121 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
      181 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
      241 tcatgtggtc ggggtagcgg ctgaagcact gcacgcccca ggtcagggtg gtcacgaggg
      301 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
      361 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
      421 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg
      481 tggcggatcc gagctcggta ccaagcttgg gtctccctat agtgagtcgt attaatttcg
      541 ataagccagt aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc
      601 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga
      661 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
      721 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcc
//
