LOCUS       sequence-118781-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses activated OTUB1 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINB-OTUB1*
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgtttaaat ggtctagaaa gctttatttg
       61 tagaggatat cgtagtgtcc aggccggtag agaaggtaga ccttgggctc ggagccctca
      121 gggaagatgt gcggattggt ggtgccgccc tcgccgcggt ccatgtactc cacctggatg
      181 gacacgctga gggcctgggc cagcgcaatg atgtggatgt ggtcgctctc cttgcacatg
      241 ggctccacct cctgctggca gaactccttg acagtccgtc caccctcgat gaagtgctcg
      301 aagaacttgc tctcgcgctg caggtagccc gaggtgagca gccgcaggta gaccacaagg
      361 tagtcggagg tgctctggtc attgaaggag gccagcaggt cggcgacaga ggtctgcttc
      421 tccacctgct caatcaggtc catgaacgtg ttgtggaaat cctcaattgt gaattcagtg
      481 aagccctggg acaccaggtc ttccttgctc ttggcagaca cagccttgaa ccgctgcaac
      541 tccttgctgt catccagcag tgcctccaag tgggagaatc cgaaagcccg atagaaacag
      601 ttgccgtcag gcctggtctt gcggatgtac gagtactttt tgtggaggtc cttgatcttc
      661 tgttgataga tgttgtcatc ttcagcatac tccttgtata ggaccgagag ctccagccgc
      721 tctgacacca gagggttctg cacagcaatc tcttgctg
//
