LOCUS       sequence-118782-         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AREL1 HECT domain in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINS-AREL1
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgtttaaat ggtctagaaa gctttatcag
       61 agcatgccaa agccctcgca accctcgctg atggccagct gcagcatcct gtgcacctct
      121 tcataggagt catatgtagg gaggcacagc tggttaaaac atgtgtgtgc agtaggcagc
      181 gtgctatggg tcggagcggc aataatctga aatgagggac agagggcggc aaagcctcca
      241 ggtggtagct gagaggagcc tgttgtgaac tgaagtagcc gagccaactc ctcctgggtc
      301 agactggaaa ccacagtcca aaaccacctc atgacctttt ctctgaaatg ccatgagcca
      361 ccaacaacta ctgcatgggc tttgaagtca gacacactga tgtctccagt cccacacatc
      421 agcagctcaa gctcattctc atcaaaaata gccaaaaggt tctcagggac caattcattc
      481 aggcctttta ggaaatgttc cacctcctct ttcacttgac tggccagccg atattgggcc
      541 agcaaattta aatagaagat tttattcgca ttggtgactg gagtttgagc tccacctgtc
      601 atgagttcta caaccttatc caattgacct gatttattat atttctcttc tgcaaagacc
      661 agctccatct cactcatgtc attgttgagg at
//
