LOCUS       sequence-118785-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. 
            coli Expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, 
            Wang R, Lynch MD
  TITLE     Scalable, two-stage, autoinduction of recombinant protein expression
            in E. coli utilizing phosphate depletion.
  JOURNAL   Biotechnol Bioeng. 2020 May 22. doi: 10.1002/bit.27440.
  PUBMED    32441815
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2020 May 22. doi: 10.1002/bit.27440."
COMMENT     Sequence Label: pSMART-Ala1
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(33..62)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      146..217
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      233..260
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 aaggatctca agaagatcct ttgattttct accgaagaaa ggcccaccag tgaaggtgag
       61 ccagtgagtt gattgcagtc cagttacgct ggagtctgag gctcgtcctg aatgatatca
      121 agcttgaatt cgtttgccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      181 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      241 ttcgggtggg cctttctgcg tttatacaca gctaacacca cgtcgtccct atctgctgcc
      301 ctaggtctat gagtggttgc tggataactc tttctgacac cttactatct tacaaatgta
      361 acaaaaaagt tatttttctg taattcgagc atgtcatgtt accccgcgag cataaaacgc
      421 gtatattcag ggagaccaca acggtttccc tctacaaata attttgttta actttcgtag
      481 aagagcactt cacacttctg gaaaaaggag atataccatg atcattgggg tgccgaagga
      541 gatcaaaaat aatgagaacc gcgtcgcgtt gaccccggga ggtgtcagcc agctgatctc
      601 taatggccat cgtgtcttag ttgaaacagg cgctggcctg ggttctggct tcgaaaacga
      661 ggcctacgaa tctgcaggtg cggaaattat tgctgatcca aaacaggtct gggatgcaga
      721 gatggtcatg aaagtgaaag aaccgctccc ggaagaatat gtctattttc gtaaaggtct
      781 ggtgctgttt acatatctgc atctggcagc tgaaccggag ctcgcacaag cccttaaaga
      841 taaaggtgtc acggccatcg catacgaaac tgtcagcgaa gggcgcacgc tgccattact
      901 gaccccgatg tcagaagtgg caggccgtat ggctgcgcag atcggcgcac agtttcttga
      961 aaaaccaaag ggcgggaagg gtattctctt agcaggagtg ccgggcgtca gtcgtgggaa
     1021 agtaactatt attggtggcg
//
