LOCUS       sequence-118789-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Const. Promoter ProD targetting RNA E. coli Low Phosphate Induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  TITLE     SUPERSEDE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCASCADE-proD
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..152)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(182..808)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     713..732
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      817..888
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      121 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccag atatcgacgt
      181 cttaagaccc actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc
      241 cgaataagaa ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata
      301 atggcggcat actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt
      361 gatcttccaa tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat
      421 tctctagtga aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact
      481 gtttttctgt aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag
      541 cacatctaaa acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag
      601 ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc
      661 gcttattttt tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt
      721 tacgggttgt taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca
      781 ctttactttt atctaatcta gacatcatcc aggcatcaaa taaaacgaaa ggctcagtcg
      841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      901 actggctcac cttcgggtgg g
//
