LOCUS       sequence-118797-         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP,
            compatible with pZT-AAVS1 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pAAVS1P-iCAG.copGFP
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    214..247
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
       61 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
      121 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
      181 taattgatta ctattaataa ctagcctgaa ttgataactt cgtataaagt atcctatacg
      241 aagttatgtc gaccgatcgt cgcgagaatt cggcgcgcca atattctcac tgactccgtc
      301 ctggagttgg atgagagata atggccttac gttgtgccag gggagggtcg ggctggattt
      361 agcaagattt accttctcca aagagcggtg ctgcagtggc acagctgccc acggaggtgg
      421 gggggtcacc gtccctggag gtgatgaaga actgtgggga tgtggcactg agggacatgg
      481 ccagtgggca cggtgggtgg gttggggttg gtcttgggga tcttggaggg cttttccagc
      541 cttcatgatt tgacgattgt atgaacatct acatggcaat tctccagctg cctgtcccag
      601 tcctactgac ccagctgtat ctctccaggc aagctcttcc accccttctg cttgcatcca
      661 gacaccatc
//
