LOCUS       sequence-118799-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP,
            compatible with pZT-AAVS1 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pAAVS1P-iCAG.copGFP
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    48..272
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(273..306)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgactg tgccttctag
       61 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      121 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
      181 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
      241 caggcatgct ggggatgcgg tgggctctat ggataacttc gtatagcata cattatacga
      301 agttatgagc tcacggggac agcccccccc caaagccccc agggatgtaa ttacgtccct
      361 cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc
      421 gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc
      481 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg
      541 gaaaaagctt taggctgaaa gagagattta gaatgacaga atcatagaac ggcctgggtt
      601 gcaaaggagc acagtgctca tccagatcca accccctgct atgtgcaggg tcatcaacca
      661 gcagcccagg ctgcccagag ccacatccag cctggccttg aatgcctgca gggatggggc
      721 atccacagcc tccttgggca acctgttcag tgcgtcacca ccctctgggg gaaaaactgc
      781 ctcctcatat ccaacccaaa cctcccctgt ctcagtgtaa agccattccc c
//
