LOCUS       sequence-118802-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To create a dox-on inducible expression of human wt-TTF-1 cDNA in 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Rice SJ, Lai SC, Wood LW, Helsley KR, Runkle EA, Winslow MM, Mu D
  TITLE     MicroRNA-33a mediates the regulation of high mobility group AT-hook 
            2 gene (HMGA2) by thyroid transcription factor 1 (TTF-1/NKX2-1).
  JOURNAL   J Biol Chem. 2013 Jun 7;288(23):16348-60. doi: 
            10.1074/jbc.M113.474643. Epub 2013 Apr 26.
  PUBMED    23625920
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.474643"; journalName: "J Biol Chem"; date: 
            "2013-06-7- 7"; volume: "288"; issue: "23"; pages: "16348-60"
COMMENT     Sequence Label: Puro-TTL-wt-TTF-1
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        30..343
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    37..55
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    73..91
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    108..126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    144..162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    215..233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    251..269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     316..340
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tttgaagatc tgggggatcg ataccgtnca gtttactccc tatcagtgat agagaacgta
       61 tgtcgagttt actccctatc agtgatagag aacgatgtcg agtttactcc ctatcagtga
      121 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
      181 ccctatcagt gatagagaac gtatgtcgag tttatcccta tcagtgatag agaacgtatg
      241 tcgagtttac tccctatcag tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga
      301 ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagaa ttaattcgag
      361 ctcggtaccc ggggatcctc cggaacttag cagtctcgag accatgtcga tgagtccaaa
      421 gcacacgact ccgttctcag tgtctgacat cttgagtccc ctggaggaaa gctacaagaa
      481 agtgggcatg gagggcggcg gcctcggggc tccgctggcg gcgtacaggc agggccaggc
      541 ggcaccgcca acagcggcca tgcagcagca cgccgtgggg caccacggcg ccgtcaccgc
      601 cgcctaccac atgacggcgg cgggggtgcc ccagctctcg cactccgccg tggggggcta
      661 ctgcaacggc aacctgggca acatgagcga gctgccgccg taccaggaca ccatgaggaa
      721 cagcgcctct ggccccggat ggtacggcgc caacccagac ccgcgcttcc
//
