LOCUS       sequence-118803-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  TITLE     de Lange Lab Vectors
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLPC-N MYC
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..34
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15..33
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      65..74
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             74..103
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tggcttatcg aaattaatac gactcactat agggagaccc aagcttggta ccgagctcgg
       61 atcagccacc atggaacaaa agttgatttc tgaagaagat ttgttaagaa agagaggatc
      121 cactagtaac ggccgccagt gtgctggaat tctgcagata tccatcacac tggcggccgc
      181 tcgagcatgc atctagaggg ccgataaaat aaaagatttt atttagtctc cagaaaaagg
      241 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
      301 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
      361 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
      421 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
      481 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
      541 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
      601 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
      661 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
      721 cccgggt
//
