LOCUS       sequence-118815-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl4D with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL4D-GFP
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(702..726)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(758..777)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(759..777)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(822..860)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 aattgggaca actccagtga aaagttcttc tcctttgcta gccattctag atcgaaccac
       61 tttgtacaag aaagctgggt cccgtctctt cttgccaccc cgagctgcct tcttcctctt
      121 gaggatcatc tcatagaggc gctcaaggcc ctgctgcagg cccagaccgt ccacagcgct
      181 gcagccttgc acatgagtga gagtggcggc tgctagctct cggactgcca gcctcttctc
      241 cacctcagca gcgctcagtg ccccgggctg gtcctgcttg ttggccagca ccagcactgg
      301 cacgccctgg ttgtccgagg cccggctgat tcggtgcaac tccaccttgg cttcctccag
      361 ccgctcagcc tccgcagcgt ccaccacaaa cactagaccg tctgtccggc gggtataaga
      421 gcgccacagt ggtcgcagct tctcctgccc cccgacgtcc cacacttgga aggtgatgcc
      481 acgcgatccc ccgaggggca cccggatctt ctcggtgttg aagcctttgg tggggacact
      541 ctggacaaac tccttgaact tgaggcggta aaggagggag gtctttccag cagagtccag
      601 cccaatgacc acgacatgca gggcttggaa gtggggcaag aaggaggagg cagtgggcgc
      661 catctcagtc aagtggttcc ccatggttct atctccttct aagcctgctt ttttgtacaa
      721 acttgtttga tcaagcttaa ctagccagct tgggtctccc tatagtgagt cgtattaatt
      781 tcgataagcc agtaagcagt gggttctcta gttagccaga gagctctgct tatatagacc
      841 tcccaccgta cacgcctacc gccc
//
