LOCUS       sequence-118818-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses UBE3C HECT domain in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINS-UBE3C
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggtagca gccatcacca tcatcatcac
       61 gggagcgata gcgaagtgaa ccaggaagcg aaaccggaag ttaaaccgga agtgaaaccg
      121 gaaacccata ttaatctgaa agttagcgac ggcagcagcg aaatcttttt taaaattaaa
      181 aaaaccaccc cgctgcgtcg cctgatggaa gcctttgcga aacgtcaggg taaagaaatg
      241 gatagcctgc gctttctgta tgacggcatc cgtattcagg ccgatcagac cccggaagac
      301 ctggatatgg aagacaacga tattattgaa gcgcatcgcg aacagatcgg tggtgttccg
      361 ttcgaagaac gcgtgaaaat ctttcagcgt ctgatctatg cagataagca ggaagtccaa
      421 ggtgatggcc cgttcctgga cggtattaac gtgaccatcc gtcgcaacta catctacgaa
      481 gatgcctatg acaaactgag tccggaaaac gaaccggatc tgaaaaagcg tatccgcgtt
      541 catctgctga atgcacacgg tctggatgaa gctggcattg acggcggtgg catctttcgt
      601 gaatttctga acgaactgct gaaatctggt tttaacccga atcagggctt tttcaagacc
      661 acgaacgaag gtctgctgta tccgaatccg gcggcccaaa tgctggtcgg cgatagcttt
      721 gcgcgccatt attacttcct gggtcgtatg ctgggcaaag cgctgtacg
//
