LOCUS       sequence-118819-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chloride-conducting Channelrhodopsin with neuron-specific promoter, 
            plus red fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Wietek J, Beltramo R, Scanziani M, Hegemann P, Oertner TG, Simon 
            Wiegert J
  TITLE     An improved chloride-conducting channelrhodopsin for light-induced 
            inhibition of neuronal activity in vivo.
  JOURNAL   Sci Rep. 2015 Oct 7;5:14807. doi: 10.1038/srep14807.
  PUBMED    26443033
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-7"; volume: "5:14807. doi"; pages: " 
            10.1038/srep14807"
COMMENT     Sequence Label: pAAV CaMKIIa iChloC 2A tDimer
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..722)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     primer_bind     complement(110..133)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(308..327)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     525..545
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     577..595
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttgtaatcca gaggttgatt atcgataagc ttttgctcga agtcgaccta caggaacagg
       61 tggtggcggc cctcggagcg ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag
      121 gtgatgtcca gcttggtgtc cacgtagtag tagccgggca gctgcacggg cttcttggcc
      181 atgtagatgg tcttgaactc caccaggtag tggccgccgt ccttcagctt cagggcctgg
      241 tggatctcgc ccttcagcac gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag
      301 cccatggtct tcttctgcat tacggggccg tcggggggga agttggtgcc gcggaacttc
      361 accttgtaga tcagcgtgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg
      421 ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcttg
      481 tagtcgggga tgtcggcggg gtgcttcacg tacgccttgg agccgtactg gaactggggg
      541 gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag cttggcggtc
      601 tgggtgccct cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc
      661 acggagccct ccatgcgcac cttgaagcgc atgaactctt tgatgacgtc ctcggaggag
      721 gcggtgccgg agctgccgct gccggtgctg ccggtgccat gcccc
//
