LOCUS       sequence-118834-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  APEX2 fused to the C-terminus human vimentin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: Vimentin - APEX2 in pECFP
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(148..171)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatct agagtcgcgg ccgcttattt gtcatcatcg tccttataat cggcgtcggc
      181 aaatcccagt tctgacagct tctgatgtgc ttctgcgtaa tctgcgaaaa aagcatcctc
      241 gtcggctgca tacttatcga ccagaggtct gaacacggga tcggacagca gggctttgtc
      301 gctgggcagc tgcagcagcc cttccttctc tccactcagc agttcagtaa agtaagaatt
      361 gtcgaaaatc agggggttgc ttgtccaggg tccctcgaag cctgaccttt ccttatgagc
      421 ggcgccaatg gtgtgccctc cagacagtgc gacgatatcc tggtcagtca ggcccatagc
      481 tttcccaaac acatctcgca gatggtcgga gccctttgtc ggatctggca ggcgcccctc
      541 tggaggtggt tctggtttgt cctcccgtcc tgggtggaaa ggcaccttgg ggcccccagt
      601 gacctccaca gcgaccactc ctgccagctg ataaaaatcg gcgtaggaca gaattgggaa
      661 ctctgctttc agaggttcca gcagccgcac ggcgatgtcc agcccattgt tggcactatg
      721 tgccagttca gcagggtgct tgattgttcc aaagggtccg cctgttttgg ttcccttgtc
      781 aaaggtgcct gcgctgtgga aagccagtct cagcatcaga ggtgcgcacc tcttttctgc
      841 gataaaccct ctcagcttct tcttggcttt ctccacggca tcctggtagt ctgcggacac
      901 tgttgggtat gatttcccgg tggcgaccgg tggatcccct tcaaggtcat cgtgatgctg
      961 agaagtttcg ttgataacct gtccatctct agtttcaacc gtcttaatca gaagtgtcct
     1021 ttttgagtgg gtatcaacca gagggagtga atcc
//
