LOCUS       sequence-118843-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase expression driven by Lin28b promoter P3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Chang TC, Zeitels LR, Hwang HW, Chivukula RR, Wentzel EA, Dews M, 
            Jung J, Gao P, Dang CV, Beer MA, Thomas-Tikhonenko A, Mendell JT
  TITLE     Lin-28B transactivation is necessary for Myc-mediated let-7 
            repression and proliferation.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3384-9. doi: 
            10.1073/pnas.0808300106. Epub 2009 Feb 11.
  PUBMED    19211792
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0808300106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-3- 3"; volume: "106"; issue: "9"; pages: "3384-9"
COMMENT     Sequence Label: pGL3-IRES-Lin28b-P3
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    98..665
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(258..277)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     487..504
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 cggagaatgg cgccgggcct ttctttatgt ttttggcgtc ttccatggtg gctttaccaa
       61 cagtaccgga atgccaagct tacttagatc gcagatctta tcatcgtgtt tttcaaagga
      121 aaaccacgtc cccgtggttc ggggggccta gacgtttttt taacctcgac taaacacatg
      181 taaagcatgt gcaccgaggc cccagatcag atcccataca atggggtacc ttctgggcat
      241 ccttcagccc cttgttgaat acgcttgagg agagccattt gactctttcc acaactatcc
      301 aactcacaac gtggcactgg ggttgtgccg cctttgcagg tgtatcttat acacgtggct
      361 tttggccgca gaggcacctg tcgccaggtg gggggttccg ctgcctgcaa agggtcgcta
      421 cagacgttgt ttgtcttcaa gaagcttcca gaggaactgc ttccttcacg acattcaaca
      481 gaccttgcat tcctttggcg agaggggaaa gacccctagg aatgctcgtc aagaagacag
      541 ggccaggttt ccgggccctc acattgccaa aagacggcaa tatggtggaa aataacatat
      601 agacaaacgc acaccggcct tattccaagc ggcttcggcc agtaacgtta gggggggggg
      661 gcggaatt
//
