LOCUS       sequence-118851-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Chang TC, Wentzel EA, Kent OA, Ramachandran K, Mullendore M, Lee KH,
            Feldmann G, Yamakuchi M, Ferlito M, Lowenstein CJ, Arking DE, Beer 
            MA, Maitra A, Mendell JT
  TITLE     Transactivation of miR-34a by p53 broadly influences gene expression
            and promotes apoptosis.
  JOURNAL   Mol Cell. 2007 Jun 8;26(5):745-52. Epub 2007 May 31.
  PUBMED    17540599
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-8"; volume: "26(5)"; pages: "745-52. Epub 2007 May 
            31"
COMMENT     Sequence Label: pGL3-Basic-IRES
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    72..639
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(233..250)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     460..479
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      685..694
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(757..775)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tttctctatc gatggtaccg agctcttacg cgtgctagcc cgggctcgag atctgacccc
       61 atgcatcgaa ttccgccccc cccccctaac gttactggcc gaagccgctt ggaataaggc
      121 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
      181 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
      241 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
      301 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
      361 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
      421 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
      481 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
      541 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
      601 cacggggacg tggttttcct ttgaaaaaca cgatgataag atctgcgatc taagtaagct
      661 tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc
      721 ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg
      781 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac
      841 atcacttacg ctg
//
