LOCUS       sequence-118852-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses activated AMSH in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINB-AMSH*
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..93
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggtc tggaagttct gtttcagggc ccgactgcca acccgttcga gcaagatgtg
      121 gaaaaagcca caaatgagta caacaccaca gaagactgga gtctgatcat ggacatctgt
      181 gacagggttg gaagcactcc cagtggagca aaagattgcc taaaagccat aatgaaaagg
      241 gtaaaccata aggtccctca cgttgctctg caggcattga cccttcttgg agcttgtgtg
      301 gcaaactgtg gaaagatatt tcatttagaa gtatgttccc gtgattttgc aacggaagta
      361 cgttctgtga taaaaaataa ggctcatcct aaagtatgtg aaaaactaaa atctctaatg
      421 gtggaatggt cagaagaatt ccagaaggac ccccaattta gtctgatatc tgcaactatt
      481 aagtctatga aagaagaagg ggttactttt ccttccgcag gctcccagac tgtcgcggct
      541 gctgccaaaa acgggacatc attgaacaaa aacaaagaag atgaggacat agctaaagct
      601 attgagttat cgttgcaaga gcagaagcag cagtacacgg agacaggcgg cagcagcggc
      661 ggcagcaact cagaaagtat tcccacaatc gatggattgc gccatgtggt ggtgcctggg
      721 cggctgtgcc cacagtttct ccagttagcc agtgccaaca ctg
//
