LOCUS       sequence-118854-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses UBE2S (1-196) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Bremm A, Freund SM, Komander D
  TITLE     Lys11-linked ubiquitin chains adopt compact conformations and are 
            preferentially hydrolyzed by the deubiquitinase Cezanne.
  JOURNAL   Nat Struct Mol Biol. 2010 Aug;17(8):939-47. doi: 10.1038/nsmb.1873. 
            Epub 2010 Jul 11.
  PUBMED    20622874
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.1873";
            journalName: "Nat Struct Mol Biol"; date: "2010-08"; volume: "17"; 
            issue: "8"; pages: "939-47"
COMMENT     Sequence Label: pOPINK-UBE2S
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..708
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     666..688
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atggcacatc accatcacca tcacatgtcc
       61 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa
      121 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga
      181 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat
      241 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg
      301 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt
      361 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt
      421 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat
      481 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt
      541 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt
      601 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct
      661 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctgagc
      721 agcggtctgg aagttctgtt tca
//
