LOCUS       sequence-118856-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses activated OTUB1 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINB-OTUB1*
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..93
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggtc tggaagttct gtttcagggc ccgatggctc tgaagagaat ccacaaggaa
      121 ttgaatgatc tggcacggga ccctccagca cagtgttcag caggtcctgt tggagatgat
      181 atgttccatt ggcaagctac aataatgggg ccaaatgaca gtccctatca gggtggagta
      241 tttttcttga caattcattt cccaacagat taccccttca aaccacctaa ggttgcattt
      301 acaacaagaa tttatcatcc aaatattaac agtaatggca gcattgctct tgatattcta
      361 cgatcacagt ggtctccagc actaactatt tcaaaagtac tcttgtccat ctgttctctg
      421 ttgtgtgatc ccaatccaga tgatccttta gtgcctgaga ttgctcggat ctacaaaaca
      481 gatagagaaa agtacaacag aatagctcgg gaatggactc agaagtatgc gatgggcggc
      541 agcagcggcg gcagcagcgg cggcagcgac tccgaaggtg ttaactgtct ggcctatgat
      601 gaagccatca tggctcagca ggaccgaatt cagcaagaga ttgctgtgca gaaccctctg
      661 gtgtcagagc ggctggagct ctcggtccta tacaaggagt atgctgaaga tgacaacatc
      721 tatcaacaga agatcaagga cctccac
//
