LOCUS       sequence-118863-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AREL1 HECT domain in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINS-AREL1
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggtagca gccatcacca tcatcatcac
       61 gggagcgata gcgaagtgaa ccaggaagcg aaaccggaag ttaaaccgga agtgaaaccg
      121 gaaacccata ttaatctgaa agttagcgac ggcagcagcg aaatcttttt taaaattaaa
      181 aaaaccaccc cgctgcgtcg cctgatggaa gcctttgcga aacgtcaggg taaagaaatg
      241 gatagcctgc gctttctgta tgacggcatc cgtattcagg ccgatcagac cccggaagac
      301 ctggatatgg aagacaacga tattattgaa gcgcatcgcg aacagatcgg tggttctgag
      361 acctttcagg acaaggtgaa ctttttccag cgagagcttc ggcaggtaca tatgaaaaga
      421 ccacattcca aagtcaccct gaaggtcagc agacatgcct tgttggaatc gtctctgaaa
      481 gccactcgga atttctccat ctcagattgg agcaagaact ttgaggttgt tttccaggat
      541 gaagaagctc tggactgggg agggcctcgc cgggaatggt ttgagctaat ctgcaaagca
      601 ctatttgata caaccaatca gctcttcacc cggttcagtg acaacaacca agcattagtg
      661 catcccaacc ctaatcgccc cgctcatctg cgcctgaaaa tgtatgagtt tgcgggacgg
      721 ctcgtgggca agtgtctcta tgagtcctct ctaggaggag cctacaagca gttggtccga
      781 gctcgcttca
//
