LOCUS       sequence-118874-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  hArl8b with stop codon in Gateway Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDONR221-ARL8B
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(695..719)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgataagc aatgctttct tataatgcca actttgtaca agaaagctgg gtcctatcag
      121 cttcttctag attttgaatg ctgaataagc cactgaagtg tgatatctat attatccttt
      181 tctttgcaag aaattgaata gcagcaaatt tctctatcct gaatagcaga cagattcatt
      241 ttttcaatta gctgtttctc atccaaggca ttaggaagat ctctcttgtt tccaagcact
      301 agcactggaa ttccttgtaa ctgtggttta tctagaagat tatgtagctc atttcgggaa
      361 gcttctatct tttcacgatc tgcagcatct atcatgtaaa caatagcatt gactcctctg
      421 caataccgct cccacatgct tcgaaatcgg ggttgtcctc ctatgtccca gatctttatt
      481 gtgacgttac ctttagttac cttcctcatg ttgaagccca ctgtgggtat catatcttca
      541 ctgaattgac ctgacgcgat gacattgacg aaggtggtct tgcccgagta ctgcagcccc
      601 acgagcgtca gctccatctc ttccttccag aagagcgaac ggaaccagtc cagcaggcgg
      661 gagatgagcg ccagcatggt tctatctcct tctaagcctg cttttttgta caaagttggc
      721 a
//
