LOCUS       sequence-118875-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 GTF2E1
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..35)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(179..198)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(180..198)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        230..334
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(553..572)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgcttgtgc cgtggtgatg gtggtgatgg tggtgaccag aggtgcccat ggttttcact
       61 tacgtgttta atgtctttac agactttata cattactgtc atggttaaat aaaataaaat
      121 aaaataaaat aaaataaaat aaaataaaat aaaataaaat aaaataaact taagatgtcc
      181 ctatagtgag tcgtattaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      241 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      301 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      361 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      421 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      481 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      541 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      601 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
      661 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
      721 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgc
//
