LOCUS       sequence-118879-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cell-free expression control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G05 sfGFP
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             119..142
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(288..306)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     556..575
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atttatttta ttttatttta ttttatttta ttttatttta ttttatttaa ccatgacagt
       61 aatgtataaa gtctgtaaag acattaaaca cgtaagtgaa accatgggca cctctggtca
      121 ccaccatcac caccatcacc acggcacaag caagggcgag gaggataaca tggccatcat
      181 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      241 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      301 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      361 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      421 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      481 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      541 cttcccctcc gacggccccg taatgcagaa gaagactatg ggctgggagg cctcctccga
      601 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
      661 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
      721 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
      781 caccatcgtg gaacagtacg aacgcgccga
//
