LOCUS       sequence-118883-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 IL2
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..32)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(175..194)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(176..194)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        226..330
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(549..568)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gctgtgccgt ggtgatggtg gtgatggtgg tgaccagagg tgcccatggt ttcacttacg
       61 tgtttaatgt ctttacagac tttatacatt actgtcatgg ttaaataaaa taaaataaaa
      121 taaaataaaa taaaataaaa taaaataaaa taaaataaaa taaacttaag atgtccctat
      181 agtgagtcgt attagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      241 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      301 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      361 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      421 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      481 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      541 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
      601 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      661 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      721 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgc
//
