LOCUS       sequence-118884-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 ISG15
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..33)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(176..195)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(177..195)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        227..331
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(550..569)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcttgtgccg tggtgatggt ggtgatggtg gtgaccagag gtgcccatgg tttcacttac
       61 gtgtttaatg tctttacaga ctttatacat tactgtcatg gttaaataaa ataaaataaa
      121 ataaaataaa ataaaataaa ataaaataaa ataaaataaa ataaacttaa gatgtcccta
      181 tagtgagtcg tattagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      241 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      301 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      361 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      421 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      481 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      541 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      601 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      661 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      721 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgc
//
