LOCUS       sequence-118891-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArfrp in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARFRP1
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(648..672)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(725..743)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      739..786
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gtttgtacaa aaagcaggct tagaaggaga tagaaccatg tacacgctgc tgtcgggctt
       61 gtacaagtac atgtttcaga aggacgagta ctgcatcctg atcctgggcc tggacaatgc
      121 tgggaagacg accttcctgg agcagtcgaa aacccgattt aacaagaact acaaggggat
      181 gagtctatcc aaaatcacca ccaccgtggg cctaaacatc ggcactgtgg atgtgggaaa
      241 ggctcggctc atgttctggg acttaggagg gcaggaagag ctgcagtctt tgtgggacaa
      301 gtattatgcg gagtgtcacg gcgtcatcta cgtcattgac tccaccgacg aggagaggct
      361 ggctgagtcc aagcaggcgt ttgagaaggt ggtgaccagc gaggcgctgt gcggtgtccc
      421 cgtcttggtg ctggccaaca agcaggatgt ggagacgtgc ctctcaatcc ctgacatcaa
      481 gacggccttc agcgactgca ccagcaagat cggcaggcgc gattgcctga cccaggcctg
      541 ctcggccctc acaggcaaag gggtgcgcga gggcatcgag tggatggtga agtgtgtcgt
      601 gcggaatgtg caccggccgc cgcggcagag ggacatcacg tagtaggacc cagctttctt
      661 gtacaaagtg gtgatgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      721 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      781 tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat
      841 cgcgtagtcg atagtggctc caagta
//
