LOCUS       sequence-118892-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl2 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL2-GFP
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    64..88
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(659..683)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(715..734)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(716..734)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(779..984)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(808..828)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttcacaagaa ttgggacaac tccagtgaaa agttcttctc ctttgctagc cattctagat
       61 cgaaccactt tgtacaagaa agctgggtcg tcagctgtga aaatgcggct ggaaatgtca
      121 tccaggagcc agtcgatgcc cggcagcagg ttctccccgg tgacggcgct gcagccctgg
      181 atgcaccagt ggtggctgcg gatggagtcc agctccaggg cctcgcggat ggcgttagag
      241 gacagtgctc caggcaggtc ctgcttatta gcaaagatga ggagggttgc tccggccagg
      301 cgctcctcca ccagcaggct ctggagctcc cgctggcagt cctgcatgcg ctggcggtct
      361 gcgctgtcca ctacccagat gaggccatcg gtgctctcaa agtagttccg ccagtaggac
      421 cgcagggact tctggccacc cacatcccag atgttcagct tgaatcctcg gtgctccagg
      481 gtcttgatgt tgaagcccag cgttggggag atggtgtcga tgtcctcccc attgaacttc
      541 ttcaggatgg ttgtctttcc agcattgtcc aggccaagca tgagcagtcg cagctcccgc
      601 tctttctgct tcatcttctt cagaatggtc aggagcccca tggttctatc tccttctaag
      661 cctgcttttt tgtacaaact tgtttgatca agcttaacta gccagcttgg gtctccctat
      721 agtgagtcgt attaatttcg ataagccagt aagcagtggg ttctctagtt agccagagag
      781 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
      841 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa actcccattg
      901 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa acccgctatc cacgcccatt
      961 gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt aaatgtactg
     1021 ccaagtagga
//
