LOCUS       sequence-118893-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl3 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL3-GFP
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(646..670)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(702..721)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(703..721)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(766..969)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(795..815)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg tctttcttct ttgcattgac atttttgcag acccagttca
      121 tgccatcctg aacgccctct cctgtgagag ctgagcaaga ctggatctgc cagactcggt
      181 cgcggatggt atgcaggttc agtccttctg caatttcaga ggcaggggct gctgtgagca
      241 aatcctgctt attagcaaag atgagcactg gcacacaact tagtttttct tcctccagta
      301 attccgctag ttcctgaccc gtctcttcaa atctttttct gtctgcactg tcgattacat
      361 atataagaat atcggtattt tcaaaataat tcttccagta tggtctgatt ttcctctgtc
      421 caccaatgtc ccatacattc agtttaaaac cttgtgattg tacacttttg atgttgaaac
      481 cctgtgtagg tgtgatgtgg ctgatgtctt cagatgcaag ctgcttcaga agagtggtct
      541 tgccagcatt atccaagccc aggagaagta ttctcacctc ctggtctggt gcacttttca
      601 acttgcgcaa aattgagagc aagcccatgg ttctatctcc ttctaagcct gcttttttgt
      661 acaaacttgt ttgatcaagc ttaactagcc agcttgggtc tccctatagt gagtcgtatt
      721 aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc tgcttatata
      781 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
      841 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg
      901 gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccatggatgt actgccaaaa
      961 ccgcatcacc atggtaatag cgatg
//
