LOCUS       sequence-118894-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl4 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL4-GFP
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(701..725)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(757..776)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(758..776)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(821..920)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(850..870)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtctcttttc tttttctgtt gccgcaacat ttttcttctt
      121 ttaatgatca tatcatgtag tttctcaagt ccttccttta ggccatctcc tatgattgca
      181 caggtaggct gcaaatgcca aggagttgat gagctcagtt cacccattgc taacaatttc
      241 tcaatttctg aaagtgacaa tgagttcctc aaatcttgtt tgttagcaac tataagtaca
      301 gggactccct gattttctga tatcctagtt attttgtgaa gttcagtttt ggcttcttcc
      361 atcctttcga catcaacaga gtccacaaca aatacaatgc catctgtgca tctggtatat
      421 gacttccaca gtggccttaa tttctcctga ccacctacat cccagaagtg aaaagtgact
      481 gttttagaat ttcccaaggt taccttaatt ttctcagtgt taaatccttt ggtaggtacg
      541 gtatttacaa attcattgaa ctgcagcctg tataagacag ttgtctttcc agcacagtcc
      601 aaacccagaa taacaatgtg gaaagactga aatgaaggca ggttggacag gatagaagtc
      661 tggtctgaca gcccattccc catggttcta tctccttcta agcctgcttt tttgtacaaa
      721 cttgtttgat caagcttaac tagccagctt gggtctccct atagtgagtc gtattaattt
      781 cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct
      841 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
      901 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg ggggggagac
      961 ttggaaatcc ccgtgagtca aaccgcta
//
