LOCUS       sequence-118895-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl5 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL5A-GFP
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(636..660)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(692..711)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(693..711)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(756..855)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(785..805)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aattgggaca actccagtga aaagttcttc tcctttgcta gccattctag atcgaaccac
       61 tttgtacaag aaagctgggt ctctaatctt aagtcgtgac atcatccatt caagtccttg
      121 gcacaatccc tcgccagtta gagcacagca tgcctggata tgccactggt gatctttaat
      181 agaagttagc ttcaaaaact gggagatttc tgctacagtc atgcattctt taacatcttg
      241 tttattagca aaaatcagca atccagcttt tcttaggtcc tcatgcgcta acattttata
      301 gagttcttct ctagttacag aaatcctctc tctgtctgta ctgtccacaa caactattac
      361 aaactctgtg ttagtatagt aagtgttcca ggaagaacga agagattctt ggccaccaat
      421 atcccacatt aggaaacgtg tattattaat cactatctct tctacattac ttcctattgt
      481 aggagatgta tgtacaactt cattcataga aaattggtaa agaatggtag ttttccctgc
      541 attatccaac ccaacaatga taactttgtg ctcctggtga ttgaacagtc tccatattct
      601 agtgaagaga attcccatgg ttctatctcc ttctaagcct gcttttttgt acaaacttgt
      661 ttgatcaagc ttaactagcc agcttgggtc tccctatagt gagtcgtatt aatttcgata
      721 agccagtaag cagtgggttc tctagttagc cagagagctc tgcttatata gacctcccac
      781 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag
      841 tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga
      901 aatccccgtg agtcaaaccg ctatccacgc
//
