LOCUS       sequence-118896-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl5b with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL5B-GFP
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(636..660)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(692..711)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(693..711)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aattgggaca actccagtga aaagttcttc tcctttgcta gccattctag atcgaaccac
       61 tttgtacaag aaagctgggt ctctcacacc aatccgggag gtcatccact ctagaccttg
      121 gcataaccct tctcctgtga gagcacagca ggattgaatg tgccatggat gatccttaat
      181 tgaactaagg gtgaggtatt tcgagatttc agctgctgtc atacaccctt tcatatcctg
      241 tttatttgca aagataagga ctgcagcctt ccgtaaatcc tcatgagcca acattctgta
      301 taattcttct tttgtaatag ctagtcgttc cctgtcaatg ctatcaacaa caagaatgat
      361 gaactctgta tttgagtaat atgtgttcca ggatgatcgc agagactcct gaccaccaat
      421 atcccacata agaaaatgag tgttcttcac aactatttct tcaacattgc ttcctatggt
      481 tggagaagta tgaaccactt cattcattaa gaattggtaa agaatggtgg ttttccctgc
      541 attatccagt cccactataa ttactttgtg ttcttggtta cagaagaggc tccacagttt
      601 ggcgaagatc agccccatgg ttctatctcc ttctaagcct gcttttttgt acaaacttgt
      661 ttgatcaagc ttaactagcc agcttgggtc tccctatagt gagtcgtatt aatttcgata
      721 agccagtaag caggtgggtt ctctagttag ccagagagct ctgcttatat agac
//
