LOCUS       sequence-118897-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl6 with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL6-GFP
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(657..681)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(713..732)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(714..732)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(777..876)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(806..826)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aattgggaca actccagtga aaagttcttc tcctttgcta gccattctag atcgaaccac
       61 tttgtacaag aaagctgggt ctgtcttcac agtctggatc tgatcttgaa gccagtctac
      121 accttcttgc aagccttctc cttttatggc atcactagca caaatatgcc agggtttatc
      181 tttgatgttc tctaaacaca gcaactgaga cacttttaca gatgtcactg catctctaag
      241 atccatttta tttgcaaaga ataagattgg aattcgacgg tgtttaatat ctggatgatt
      301 cagaagagta tcgagttctt ctttggccac aaccattctt aatctatcac tactatcaat
      361 gacaaaaata atagcttggc cttctttata atagtgttcc cagagatttc tgtatcttcc
      421 ttgacctgac atgtcaaaca ctgtaaatga caaactggat gatttgaatt tctctatgct
      481 gaatcctatt gttggaagga tattttgaga ttgagcattt gaaggtttaa gtttgttaat
      541 gatcgtcgtt ttgccactat tatctagccc aaggcacaaa acatgaacct ccttcttctt
      601 caggccaagc aagactgaaa gtctgtctag caatcccatg gttctatctc cttctaagcc
      661 tgcttttttg tacaaacttg tttgatcaag cttaactagc cagcttgggt ctccctatag
      721 tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct
      781 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
      841 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
      901 tcaatggggt ggaga
//
