LOCUS       sequence-118899-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl8b with C-term GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARL8B-GFP
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(658..682)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(714..733)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(715..733)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(778..877)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(807..827)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcgcttctt ctagattttg aatgctgaat aagccactga
      121 agtgtgatat ctatattatc cttttctttg caagaaattg aatagcagca aatttctcta
      181 tcctgaatag cagacagatt cattttttca attagctgtt tctcatccaa ggcattagga
      241 agatctctct tgtttccaag cactagcact ggaattcctt gtaactgtgg tttatctaga
      301 agattatgta gctcatttcg ggaagcttct atcttttcac gatctgcagc atctatcatg
      361 taaacaatag cattgactcc tctgcaatac cgctcccaca tgcttcgaaa tcggggttgt
      421 cctcctatgt cccagatctt tattgtgacg ttacctttag ttaccttcct catgttgaag
      481 cccactgtgg gtatcatatc ttcactgaat tgacctgacg cgatgacatt gacgaaggtg
      541 gtcttgcccg agtactgcag ccccacgagc gtcagctcca tctcttcctt ccagaagagc
      601 gaacggaacc agtccagcag gcgggagatg agcgccagca tggtttatct ccttctaagc
      661 ctgctttttt gtacaaactt gtttgatcaa gcttaactag ccagcttggg tctccctata
      721 gtgagtcgta ttaatttcga taagccagta agcagtgggt tctctagtta gccagagagc
      781 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
      841 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
      901 gtcaatgggg gtggagactg ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
      961 tgtactg
//
