LOCUS       sequence-118900-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 SP2
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..29)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(172..191)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(173..191)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        223..327
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(546..565)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtgccgtggt gatggtggtg atggtggtga ccagaggtgc ccatggtttc acttacgtgt
       61 ttaatgtctt tacagacttt atacattact gtcatggtta aataaaataa aataaaataa
      121 aataaaataa aataaaataa aataaaataa aataaaataa acttaagatg tccctatagt
      181 gagtcgtatt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      241 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      301 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      361 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      421 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      481 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
      541 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
      601 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
      661 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
      721 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
//
