LOCUS       sequence-118901-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hArf3 with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-ARF3-V5-His6
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(53..94)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    114..138
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(700..724)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    765..789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(789..808)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(790..808)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttgttagca gccggatcaa actcaatggt gatggtgatg atgaccggta cgcgtagaat
       61 cgagaccgag gagagggtta gggataggct taccttcaag cttcgaattg atcaccactt
      121 tgtacaagaa agctgggtcc ttcttgtttt tgagctgatt ggccagccag tccaggcctt
      181 cgtacagccc gtccccgctg gtggcacagg tggcctgaat gtaccagtta cggtgacgaa
      241 gggaatgcag gcccagcttg tctgtgatct cagcagcgtt catagcatta ggcagatcct
      301 gtttgtttgc aaagacaagg agtacagcat cccggagctc gtcctccgcc agcattctca
      361 tcagctcttc ccgggcctca tttactcgct cccgatcatt gctgtcgacc acaaatatca
      421 acccttgggt gttctggaag tagtgtctcc agaggggtcg aatcttgtcc tggccaccca
      481 catcccacac tgtaaagctg atgttcttat actccactgt ctccacattg aacccaatgg
      541 tagggatggt ggtgacgatc tcccccagtt tcagcttgta taggatggtg gtctttcctg
      601 cggcatccag gcccaccatc aggatgcgca tctccttctt cccaatcagg ctcttgagaa
      661 ggtttccaaa gatattgccc atggttctat ctccttctaa gcctgctttt ttgtacaaac
      721 ttgtgatatt ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct
      781 cacaattccc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atccctctac
      841 gccggacgca tcgtggccgg catcaccggg cgccacaggg tgcggttgct ggcgcctata
      901 tcgccgaaaa tcaccgatgg ggaaa
//
