LOCUS       sequence-118910-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TNF
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..35)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(178..197)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(179..197)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        229..333
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(552..571)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgcttgtgc cgtggtgatg gtggtgatgg tggtgaccag aggtgcccat ggtttcactt
       61 acgtgtttaa tgtctttaca gactttatac attactgtca tggttaaata aaataaaata
      121 aaataaaata aaataaaata aaataaaata aaataaaata aaataaactt aagatgtccc
      181 tatagtgagt cgtattagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      241 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      301 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      361 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      421 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      481 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
      541 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
      601 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
      661 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
      721 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgc
//
