LOCUS       sequence-118911-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector attaches CFP(159-238) to the N-terminus of a protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: CFP(159-238) in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(47..68)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      250..259
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(290..309)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(291..309)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(354..557)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(383..403)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atctcttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
       61 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
      121 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      181 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagtgg gccttgatgc
      241 cgttcttgcc catggtggcg gatccgagct cggtaccaag cttgggtctc cctatagtga
      301 gtcgtattaa tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg
      361 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
      421 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
      481 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
      541 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
      601 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
      661 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
      721 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
      781 acatacgtca ttattgacgt caatgggcgg gggtcgttg
//
