LOCUS       sequence-118919-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This G protein alpha-q construct contains internal insertions of CFP
            and the EE epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Hughes TE, Zhang H, Logothetis DE, Berlot CH
  TITLE     Visualization of a functional Galpha q-green fluorescent protein 
            fusion in living cells. Association with the plasma membrane is 
            disrupted by mutational activation and by elimination of 
            palmitoylation sites, but not be activation mediated by receptors or
            AlF4-.
  JOURNAL   J Biol Chem. 2001 Feb 9;276(6):4227-35. Epub 2000 Nov 13.
  PUBMED    11076942
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-02-9"; volume: "276(6)"; pages: "4227-35. Epub 
            2000 Nov 13"
COMMENT     Sequence Label: G protein alpha-q-CFP
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(643..664)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct
      121 ggaattctgc agatatccat cacactggcg gccgccaccg cggtgaagct ccgcgccggc
      181 ggggctgcag cgaggcactt cggaagaatg actctggagt ccatcatggc gtgctgcctg
      241 agcgaggagg ccaaggaagc ccggaggatc aacgacgaga tcgagcggca gctgcgcagg
      301 gacaagcgcg acgcccgccg ggaactcaag ctgctgctgc tggggacagg ggagagtggc
      361 aagagcacct tcatcaagca gatgaggatc atccacgggt cgggctactc tgacgaagac
      421 aagcgcggct tcaccaagct ggtgtatcag aacatcttca cggccatgca ggccatgatc
      481 agagcgatgg acacactcaa gatcccatac aagtatgaac acaataaggc tcatgcacaa
      541 ttggttcgag aggttgatgt ggagaaggtg tctgcttttt ctggtggagg tggatccatg
      601 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      661 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      721 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      781 gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaa
//
