LOCUS       sequence-118922-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G protein alpha-s internally tagged with EGFP and EE epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Hynes TR, Mervine SM, Yost EA, Sabo JL, Berlot CH
  TITLE     Live cell imaging of Gs and the beta2-adrenergic receptor 
            demonstrates that both alphas and beta1gamma7 internalize upon 
            stimulation and exhibit similar trafficking patterns that differ 
            from that of the beta2-adrenergic receptor.
  JOURNAL   J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5.
  PUBMED    15297467
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-15"; volume: "279(42)"; pages: "44101-12. 
            Epub 2004 Aug 5"
COMMENT     Sequence Label: G protein alpha-s-GFP
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      270..279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(555..576)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagcttg gtcgaccaat tccgcagcag cagctcccgc ggctcctgct
      121 ctgctccgcc tcggccccgg agcgaggggc ggagagccgc gcgcttgcct tagtccgagc
      181 cgccaccctc cccgcgttcc ccgctttccc ggcccgcccg aggccgcccg ccccgtcccc
      241 gccgccccgc agcccggccg cgccccgccg ccgccatggg ctgcctcggc aacagtaaga
      301 ccgaggacca gcgcaacgag gagaaggcgc agcgcgaggc caacaaaaag atcgagaagc
      361 agctgcagaa ggacaagcag gtctaccggg ccacgcaccg cctgctgctg ctgggtgctg
      421 gagagtctgg caaaagcacc attgtgaagc agatgaggat tctacatgtt aatgggttta
      481 acggagaggg atccggtggg ggcgggagca tggtgagcaa gggcgaggag ctgttcaccg
      541 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      601 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      661 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      721 gcttc
//
