LOCUS       sequence-118924-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G protein alpha-s internally tagged with mCerulean and EE epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Hynes TR, Mervine SM, Yost EA, Sabo JL, Berlot CH
  TITLE     Live cell imaging of Gs and the beta2-adrenergic receptor 
            demonstrates that both alphas and beta1gamma7 internalize upon 
            stimulation and exhibit similar trafficking patterns that differ 
            from that of the beta2-adrenergic receptor.
  JOURNAL   J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5.
  PUBMED    15297467
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-15"; volume: "279(42)"; pages: "44101-12. 
            Epub 2004 Aug 5"
COMMENT     Sequence Label: G protein alpha-s-mCerulean
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      269..278
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(554..575)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctctctggct actagagaac ccactgcttt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg tcgaccaatt ccgcagcagc agctcccgcg gctcctgctc
      121 tgctccgcct cggccccgga gcgaggggcg gagagccgcg cgcttgcctt agtccgagcc
      181 gccaccctcc ccgcgttccc cgctttcccg gcccgcccga ggccgcccgc cccgtccccg
      241 ccgccccgca gcccggccgc gccccgccgc cgccatgggc tgcctcggca acagtaagac
      301 cgaggaccag cgcaacgagg agaaggcgca gcgcgaggcc aacaaaaaga tcgagaagca
      361 gctgcagaag gacaagcagg tctaccgggc cacgcaccgc ctgctgctgc tgggtgctgg
      421 agagtctggc aaaagcacca ttgtgaagca gatgaggatt ctacatgtta atgggtttaa
      481 cggagaggga tccggtgggg gcgggagcat ggtgagcaag ggcgaggagc tgttcaccgg
      541 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      601 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      661 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg
      721 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttc
//
