LOCUS       sequence-118928-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A carboxyl-terminal YFP fragment was fused to Ggamma-10. When 
            co-expressed with an amino-terminal YFP fragment fused to a Gbeta 
            subunit with which it interacts, a fluorescent signal is produced. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: YFP(159-238)-gamma-10 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(268..289)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      471..480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(511..530)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(512..530)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(575..778)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(604..624)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgtgatagat cttcagagta aagcacagga tctaggctcc cggaaggggt tacttccagc
       61 tggaacaccc accagcaggg catccttgca ggcattctgc atacagtact gttgaagctc
      121 tgcagctgcc tgagagacct tgatcctctc cacgccagcc tccaacttga gctgctctac
      181 caagcgctgc agggcgctcg cgctagcccc ggaggacatg gatctcttgt acagctcgtc
      241 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
      301 ctcgttgggg tctttgctca gggcggactg gtagctcagg tagtggttgt cgggcagcag
      361 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
      421 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttgc ccatggtggc
      481 ggatccgagc tcggtaccaa gcttgggtct ccctatagtg agtcgtatta atttcgataa
      541 gccagtaagc agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc
      601 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
      661 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
      721 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
      781 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
      841 tgtactgggc ataatgccag gcgggccatt taccgtcatt
//
