LOCUS       sequence-118929-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A carboxyl-terminal YFP fragment was fused to Ggamma-12. When 
            co-expressed with an amino-terminal YFP fragment fused to a Gbeta 
            subunit with which it interacts, a fluorescent signal is produced..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH
  TITLE     Analysis of G protein betagamma dimer formation in live cells using 
            multicolor bimolecular fluorescence complementation demonstrates 
            preferences of beta1 for particular gamma subunits.
  JOURNAL   Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26.
  PUBMED    16641313
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Pharmacol."; date: "2006-07"; volume: "70(1)"; pages: "194-205. Epub
            2006 Apr 26"
COMMENT     Sequence Label: YFP(159-238)-gamma-12 in pcDNAI/Amp
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(318..339)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      521..530
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(561..580)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(562..580)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(625..724)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(654..674)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgatagatct gcgttgttgg gaagaggcga ggagctgttt ctctattcca ctataagatg
       61 atgcaagttt ttttatcctt gaaagggttt tctgaagttg gtattcctat cagcaaaggg
      121 tcactcctgg catgttcctc acagtaggac atgaggtccg ctgatgcctt cgaaaccttt
      181 attctttcaa tggaggcttc taatcttaac tgctgcacag ttctccttgc ctgggctata
      241 ttgttggtgc ttgctgtttt gctggacatg gatctcttgt acagctcgtc catgccgaga
      301 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
      361 tctttgctca gggcggactg gtagctcagg tagtggttgt cgggcagcag cacggggccg
      421 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
      481 ttgtggcgga tcttgaagtt caccttgatg ccgttcttgc ccatggtggc ggatccgagc
      541 tcggtaccaa gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc
      601 agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct
      661 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
      721 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
      781 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cg
//
