LOCUS       sequence-118934-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A2A-adenosine receptor tagged with HaloTag on amino terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  TITLE     Berlot Lab HaloTag Fusions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HaloTag-A2A-adenosine receptor
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          125..257
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     240..259
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        302..320
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      340..349
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     346..423
                     /codon_start=1
                     /product="human growth hormone signal sequence"
                     /label=hGH signal sequence
                     /translation="MATGSRTSLLLAFGLLCLPWLQEGSA"
ORIGIN
        1 ccgtcagatc actagaagct ttattgcggt agtttatcac agttaaattg ctaacgcagt
       61 cagtgcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt gaggcactgg
      121 gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc
      181 gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt
      241 tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag gctagagtac
      301 ttaatacgac tcactatagg ctagccagct ggcgcggatg ccaccatggc tacaggctcc
      361 cggacgtccc tgctcctggc ttttggcctg ctctgcctgc cctggcttca agagggcagt
      421 gccttcccaa ccattccctt atccggatcc gaaatcggta caggcttccc cttcgacccc
      481 cattatgtgg aagtcctggg cgagcgtatg cactacgtcg atgttggacc gcgggatggc
      541 acgcctgtgc tgttcctgca cggtaacccg acctcgtcct acctgtggcg caacatcatc
      601 ccgcatgtag caccgagtca tcggtgcatt gctccagacc tgatcgggat gggaaaatcg
      661 gacaaaccag acctcgatta tttcttcgac gaccacgtcc gctacctcga tgccttcatc
      721 gaagccttgg gtttggaaga ggtcgtcctg gtcatccacg actggggctc agctctcgga
      781 ttccactggg ccaagcgcaa tccggaacgg gtcaaaggta ttg
//
