LOCUS       sequence-118941-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G protein gamma-2 tagged with HaloTag on amino terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  TITLE     Berlot Lab HaloTag Fusions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HaloTag-G protein gamma-2
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          126..258
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     241..260
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        303..321
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      344..353
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccggtcagat cactagaagc tttattgcgg tagtttatca cagttaaatt gctaacgcag
       61 tcagtgcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg tgaggcactg
      121 ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt
      181 cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact gacatccact
      241 ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa ggctagagta
      301 cttaatacga ctcactatag gctagccagc tggcgcggat atcgccacca tgggatccga
      361 aatcggtaca ggcttcccct tcgaccccca ttatgtggaa gtcctgggcg agcgtatgca
      421 ctacgtcgat gttggaccgc gggatggcac gcctgtgctg ttcctgcacg gtaacccgac
      481 ctcgtcctac ctgtggcgca acatcatccc gcatgtagca ccgagtcatc ggtgcattgc
      541 tccagacctg atcgggatgg gaaaatcgga caaaccagac ctcgattatt tcttcgacga
      601 ccacgtccgc tacctcgatg ccttcatcga agccttgggt ttggaagagg tcgtcctggt
      661 catccacgac tggggctcag ctctcggatt ccactgggcc aagcgcaatc cggaacgggt
      721 caaaggtatt gcatgtatgg aattcatccg gcctatcccg acgtgggacg aatggccaga
      781 attcgcccgt gagaccttcc aggccttccg gaccgccgac gtc
//
