LOCUS       sequence-118943-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  G protein gamma-1 tagged with HaloTag on amino terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  TITLE     Berlot Lab HaloTag Fusions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HaloTag-G protein gamma-1
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          125..257
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     240..259
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        302..320
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      343..352
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccgtcagatc actagaagct ttattgcggt agtttatcac agttaaattg ctaacgcagt
       61 cagtgcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt gaggcactgg
      121 gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc
      181 gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt
      241 tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag gctagagtac
      301 ttaatacgac tcactatagg ctagccagct ggcgcggata tcgccaccat gggatccgaa
      361 atcggtacag gcttcccctt cgacccccat tatgtggaag tcctgggcga gcgtatgcac
      421 tacgtcgatg ttggaccgcg ggatggcacg cctgtgctgt tcctgcacgg taacccgacc
      481 tcgtcctacc tgtggcgcaa catcatcccg catgtagcac cgagtcatcg gtgcattgct
      541 ccagacctga tcgggatggg aaaatcggac aaaccagacc tcgattattt cttcgacgac
      601 cacgtccgct acctcgatgc cttcatcgaa gccttgggtt tggaagaggt cgtcctggtc
      661 atccacgact ggggctcagc tctcggattc cactgggcca agcgcaatcc ggaacgggtc
      721 aaaggtattg catgtatgga attcatccgg cctatcccga cgtgggacga atggccagaa
      781 ttcgcccgtg agaccttcca ggccttccgg accgccgacg tcggccgaga gttgatcatc
      841 gatcagaacg ctttcatcga gggtgcgctc ccgat
//
