LOCUS       sequence-118947-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArf1 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARF1
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(486..510)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(563..581)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      577..624
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggtgggcctg gatgctgcag ggaagaccac gatcctctac aagcttaagc tgggtgagat
       61 cgtgaccacc attcccacca taggcttcaa cgtggaaacc gtggagtaca agaacatcag
      121 cttcactgtg tgggacgtgg gtggccagga caagatccgg cccctgtggc gccactactt
      181 ccagaacaca caaggcctga tcttcgtggt ggacagcaat gacagagagc gtgtgaacga
      241 ggcccgtgag gagctcatga ggatgctggc cgaggacgag ctccgggatg ctgtcctcct
      301 ggtgttcgcc aacaagcagg acctccccaa cgccatgaat gcggccgaga tcacagacaa
      361 gctggggctg cactcactac gccacaggaa ctggtacatt caggccacct gcgccaccag
      421 cggcgacggg ctctatgaag gactggactg gctgtccaat cagctccgga accagaagtg
      481 ataggaccca gctttcttgt acaaagtggt gatgatccgg ctgctaacaa agcccgaaag
      541 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      601 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac
      661 gggtgtggtc gccatgatcg cgtagtcgat agtggctccc aagtagcgaa
//
