LOCUS       sequence-118948-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArf3 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARF3
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(577..601)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(654..672)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      668..715
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aagcaggctt agaaggagat agaaccatgg gcaatatctt tggaaacctt ctcaagagcc
       61 tgattgggaa gaaggagatg cgcatcctga tggtgggcct ggatgccgca ggaaagacca
      121 ccatcctata caagctgaaa ctgggggaga tcgtcaccac catccctacc attgggttca
      181 atgtggagac agtggagtat aagaacatca gctttacagt gtgggatgtg ggtggccagg
      241 acaagattcg acccctctgg agacactact tccagaacac ccaagggttg atatttgtgg
      301 tcgacagcaa tgatcgggag cgagtaaatg aggcccggga agagctgatg agaatgctgg
      361 cggaggacga gctccgggat gctgtactcc ttgtctttgc aaacaaacag gatctgccta
      421 atgctatgaa cgctgctgag atcacagaca agctgggcct gcattccctt cgtcaccgta
      481 actggtacat tcaggccacc tgtgccacca gcggggacgg gctgtacgaa ggcctggact
      541 ggctggccaa tcagctcaaa aacaagaagt gataggaccc agctttcttg tacaaagtgg
      601 tgatgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      661 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      721 aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga
      781 tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaaagcg gtcggacagt
      841 gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca
//
