LOCUS       sequence-118950-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of native hArf5 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST14-ARF5
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(577..601)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(654..672)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      668..715
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgggcctcac cgtgtccgct ctcttttcgc
       61 ggatcttcgg gaagaagcag atgcggattc tcatggttgg cttggatgcg gctggcaaga
      121 ccacaatcct gtacaaactg aagttggggg agattgtcac caccatccca accataggct
      181 tcaatgtaga aacagtggaa tataagaaca tctgtttcac agtctgggac gtgggaggcc
      241 aggacaagat tcggcctctg tggcggcact acttccagaa cactcagggc ctcatctttg
      301 tggtggacag taatgaccgg gagcgggtcc aagaatctgc tgatgaactc cagaagatgc
      361 tgcaggagga cgagctgcgg gatgcagtgc tgctggtatt tgccaacaag caggacatgc
      421 ccaacgccat gcccgtgagc gagctgactg acaagctggg gctacagcac ttacgcagcc
      481 gcacgtggta tgtccaggcc acctgcgcca cccaaggcac aggtctgtac gatggtctgg
      541 actggctgtc ccacgagctg tcaaagcgct aataggaccc agctttcttg tacaaagtgg
      601 tgatgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      661 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      721 aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga
      781 tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaaagcg gtcggaacag
      841 tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagca
//
